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ND7.225 Intelligent Imaging Marianas system based on Zeiss Axiovert 200M with Roper DualView and QuantEM camera for simultaneous two-color TIRF and wide-field epifluorescence of CFP, YFP, GFP, RFP. Frequency domain FLIM with 440, 488, 532 and 561 nm lasers. Slidebook software for instrument control and image analysis. Temperature control. CO2 incubator located in room.