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K1.A06 Designed for viewing thicker specimens ranging up to a hundred µm or more (depending on turbidity). Visible laser lines: 458, 488, 514, 543 and 633 nm for conventional confocal applications. Spectral detection for optimized CFP/YFP FRET and spectral unmixing of closely overlapping fluorophores (e.g. GFP and YFP, multiplexed Qdots). Software tunable NIR laser for multiphoton excitation from 705 to 980 nm. Non-descanned detectors for deep imaging and second harmonic generation (SHG) imaging of unstained collagen. Can excite UV fluorophores down to 350 nm (e.g. DAPI). Photoablation, photoactivation, FRAP, uncaging. Fluorescent proteins in tissue/whole animal preparations. DIC optics. Environmental control including CO2.