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K1.300 For viewing fluorophores at interface between specimen and supporting coverglass. Screens out signal from deeper in the specimen. Rapid dynamics and improved signal to noise ratio relative to wide-field epifluorescence. 488, 543 and 633 nm laser excitation. DualView beamsplitter for simultaneous red/green acquisition. Photometrics CascadeII:512 EMCCD or Hamamatsu Orca II ERG CCD camera. Not readily applicable to embedded specimens. DIC optics. Temperature control. Especially recommended for imaging plasma membrane events and for single molecule imaging.