helpful stuff

Imaging Do's and Don'ts
The good, the bad and the ugly

What's in a picture? The temptation of image manipulation

Seeing is believing? A beginner's guide to practical pitfalls in image acquisition

Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images

Fluorescent Proteins
A guide to choosing fluorescent proteins

Interactive fluorescent protein properties

Interactive photoswitchable protein properties

FRET (Very important reads !!)
Fanciful FRET

FRET Standards


Colocalization of fluorophores
A guided tour into subcellular colocalization analysis in light microscopy

Quantitative Fluorescence Co-localization

Quantum Dots
Potentials and pitfalls of fluorescent quantum dots for biological imaging

Advances in fluorescence imaging with quantum dot probes

Total internal reflection fluorescence microscopy: technical innovations and novel applications

Overcoming Optical Challenges to Live-Cell TIRF Microscopy

A New Wave of Cellular Imaging

Navigating challenges in the application of superresolution microscopy 

Structured Illumination

Sample Preparation
Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours

Please submit links for suggested papers to Kate Luby-Phelps.