Text Box: The Blount Laboratory


Below is a list of publications. For more recent additions, you may also want to check here.

Recent Reviews and Book Chapters:


Iscla I, Blount P. Sensing and Responding to Membrane Tension: The Bacterial MscL Channel as a Model System. Biophys J. 2012 Jul 18;103(2):169-74. Epub 2012 Jul 17. PubMed PMID: 22853893.

Booth IR, Blount P. The MscS and MscL Families of Mechanosensitive Channels Act as Microbial Emergency Release Valves. J Bacteriol. 2012 Sep;194(18):4802-9. Epub 2012 Jun 8. PubMed PMID: 22685280; PubMed Central PMCID: PMC3430326. 

Blount P, Li Y, Moe PC, Iscla I. Mechanosensitive ion channels (a volume in the Mechanosensitivity in Cells and Tissues, Moscow Academia series). Kamkin A, Kiseleva I, editors. New York: Springer Press; 2008. Mechanosensitive channels gated by membrane tension: Bacteria and beyond; p.71-101.

Blount P, Iscla I, Li Y. Sensing with ion channels. Martinac B editor. Berlin: Springer-Verlag Press; 2008. Mechanosensitive channels and sensing osmotic stimuli in bacteria; p.25-47.

Blount P, Iscla I, Moe PC, Li Y. Mechanosensitive Ion Channels (Volume 58 in the Current Topics in Membranes series). Hamill OP editor. St. Louis: Elsievier Press; 2007. MscL: The bacterial mechanosensitive channel of large conductance; p.202-233.


Primary Publications:


Iscla I, Wray R, Blount P. The dynamics of protein-protein interactions between domains of MscL at the cytoplasmic-lipid interface. Channels (Austin). 2012 Jul 1;6(4). [Epub ahead of print] PubMed PMID: 22874845.

Yang LM, Wray R, Parker J, Wilson D, Duran RS, Blount P. Three routes to modulate the pore size of the MscL channel/nanovalve. ACS Nano. 2012 Feb 28;6(2):1134-41. Epub 2012 Jan 6. PubMed PMID: 22206349; PubMed Central PMCID: PMC3289768.

Iscla I, Wray R, Blount P. The oligomeric state of the truncated mechanosensitive channel of large conductance shows no variance in vivo. Protein Sci. 2011 Sep;20(9):1638-42. doi: 10.1002/pro.686. Epub 2011 Jul 19. PubMed PMID: 21739498; PubMed Central PMCID: PMC3190158.

Iscla I, Wray R, Blount P. An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel. FASEB J. 2011 Feb;25(2):694-702. Epub 2010 Nov 10. PubMed PMID: 21068398; PubMed Central PMCID: PMC3023395.

Yang LM, Blount P. Manipulating the permeation of charged compounds through the MscL nanovalve. FASEB J. 2011 Jan;25(1):428-34. Epub 2010 Oct 7. PubMed PMID: 20930114; PubMed Central PMCID: PMC3005423.

Dorwart MR, Wray R, Brautigam CA, Jiang Y, Blount P. S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins. PLoS Biol. 2010 Dec 7;8(12):e1000555. PubMed PMID: 21151884; PubMed Central PMCID: PMC2998437.

Li Y, Wray R, Eaton C, Blount P. An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating. FASEB J. 2009 Jul;23(7):2197-204. Epub 2009 Mar 4. PubMed PMID: 19261722; PubMed Central PMCID: PMC2704598.

Iscla I, Wray R, Blount P. On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface. Biophys J. 2008 Sep;95(5):2283-91. Epub 2008 May 30. PubMed PMID: 18515388; PubMed Central PMCID: PMC2517020.

Andersson M, Okeyo G, Wilson D, Keizer H, Moe P, Blount P, Fine D, Dodabalapur A, Duran RS. Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane. Biosens Bioelectron. 2008 Jan 18;23(6):919-23. Epub 2007 Oct 3. PubMed PMID: 17996439.

Iscla I, Levin G, Wray R, Blount P. Disulfide trapping the mechanosensitive channel MscL into a gating-transition state. Biophys J. 2007 Feb 15;92(4):1224-32. Epub 2006 Nov 17. PubMed PMID: 17114217; PubMed Central PMCID: PMC1783893.

Bartlett JL, Li Y, Blount P. Mechanosensitive channel gating transitions resolved by functional changes upon pore modification. Biophys J. 2006 Nov 15;91(10):3684-91. Epub 2006 Aug 25. PubMed PMID: 16935962; PubMed Central PMCID: PMC1630475.

Moe P, Blount P. Assessment of potential stimuli for mechano-dependent gating of MscL: effects of pressure, tension, and lipid headgroups. Biochemistry. 2005 Sep 13;44(36):12239-44. PubMed PMID: 16142922.

Folgering JH, Moe PC, Schuurman-Wolters GK, Blount P, Poolman B. Lactococcus lactis uses MscL as its principal mechanosensitive channel. J Biol Chem. 2005 Mar 11;280(10):8784-92. Epub 2004 Dec 21. PubMed PMID: 15613476.

Edwards MD, Li Y, Kim S, Miller S, Bartlett W, Black S, Dennison S, Iscla I, Blount P, Bowie JU, Booth IR. Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel. Nat Struct Mol Biol. 2005 Feb;12(2):113-9. Epub 2005 Jan 23. PubMed PMID: 15665866.

Iscla I, Levin G, Wray R, Reynolds R, Blount P. Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites. Biophys J. 2004 Nov;87(5):3172-80. Epub 2004 Aug 31. PubMed PMID: 15339809; PubMed Central PMCID: PMC1304787.

Bartlett JL, Levin G, Blount P. An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating. Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10161-5. Epub 2004 Jun 28. PubMed PMID: 15226501; PubMed Central PMCID: PMC454182.

Li Y, Wray R, Blount P. Intragenic suppression of gain-of-function mutations in the Escherichia coli mechanosensitive channel, MscL. Mol Microbiol. 2004 Jul;53(2):485-95. PubMed PMID: 15228529.

Kung C, Blount P. Channels in microbes: so many holes to fill. Mol Microbiol. 2004 Jul;53(2):373-80. Review. PubMed PMID: 15228520.

Levin G, Blount P. Cysteine scanning of MscL transmembrane domains reveals residues critical for mechanosensitive channel gating. Biophys J. 2004 May;86(5):2862-70. PubMed PMID: 15111403; PubMed Central PMCID: PMC1304155.

Blount P. Molecular mechanisms of mechanosensation: big lessons from small cells. Neuron. 2003 Mar 6;37(5):731-4. Review. PubMed PMID: 12628164.

Li Y, Moe PC, Chandrasekaran S, Booth IR, Blount P. Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli. EMBO J. 2002 Oct 15;21(20):5323-30. PubMed PMID: 12374733; PubMed Central PMCID: PMC129069.

Kumánovics A, Levin G, Blount P. Family ties of gated pores: evolution of the sensor module. FASEB J. 2002 Oct;16(12):1623-9. PubMed PMID: 12374785.

Okada K, Moe PC, Blount P. Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis. J Biol Chem. 2002 Aug 2;277(31):27682-8. Epub 2002 May 15. PubMed PMID: 12015316.

Poolman B, Blount P, Folgering JH, Friesen RH, Moe PC, van der Heide T. How do membrane proteins sense water stress? Mol Microbiol. 2002 May;44(4):889-902. Review. PubMed PMID: 12010487.

Moe PC, Levin G, Blount P. Correlating a protein structure with function of a bacterial mechanosensitive channel. J Biol Chem. 2000 Oct 6;275(40):31121-7. PubMed PMID: 10846181.

Yoshimura K, Batiza A, Schroeder M, Blount P, Kung C. Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys J. 1999 Oct;77(4):1960-72. PubMed PMID: 10512816; PubMed Central PMCID: PMC1300477.

Blount P, Moe PC. Bacterial mechanosensitive channels: integrating physiology, structure and function. Trends Microbiol. 1999 Oct;7(10):420-4. Review. PubMed PMID: 10498951.

Blount P, Sukharev SI, Moe PC, Martinac B, Kung C. Mechanosensitive channels of bacteria. Methods Enzymol. 1999;294:458-82. Review. PubMed PMID: 9916243.

Ou X, Blount P, Hoffman RJ, Kung C. One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11471-5. PubMed PMID: 9736761; PubMed Central PMCID: PMC21667.

Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. PubMed PMID: 9632260.

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