Publications on Sperm Stem Cell Biology and Technology

Izsvak Z., Frohlich F., Grabundzija I., Shirley, J.R., Powell H.M., Chapman K., Ivics Z., and Hamra F.K. (2010) Generating Knockout Rats by Sleeping Beauty Transposon Mutagenesis in Spermatogonial Stem Cells.  Nature Methods, 2010 Jun;7(6):443-5.  PMID: 20473302

Richardson T.E., Chapman K.M., Dann C.T., Hammer R.E. and Hamra F.K. (2009) Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission.  PLoS ONE, Jul 21;4(7):e6308. PMID: 19621088
“Presents a Novel System to Maximize Germline Transmission From Donor Stem Cells in the Rat”

Wu Z., Falciatori I., Molyneux L.A., Richardson T.E., Chapman K.M. and Hamra, F.K. (2009) Spermatogonial Culture Medium: an effective and efficient nutrient mixture for culturing rat spermatogonial stem cellsBiol. Reprod., Jul;81(1):77-86.  PMID: 19299316
“Reports the Formula of New and Simplified Medium for Culturing Rat Spermatogonia”

Hao J., Yamamoto M., Chapman K.M., Richardson T.E., Denard B.S., Hammer R.E., Zhao G.Q. and Hamra F.K. (2008) Sohlh2 knockout mice are male-sterile because of degeneration of differentiating type A spermatogonia. Stem Cells, Jun;26(6):1587-97. PMID: 18339773.
“Functional Analysis of a Germ Cell-Specific Transcription Factor Required for Spermatogonia to Differentiate into Spermatocytes"

Hamra, F.K., Chapman, K.M., Wu, Z. and Garbers, D.L. (2008) Isolating highly pure rat spermatogonial stem cells in culture. Methods in Molecular Biology, Humana Press, New Jersey. 450:163-178.  PMID: 18370059
Details on a Cell Culture-Based Method to Isolate Sperm Stem Cells

Hamra, F.K., Chapman, K.M., Nguyen, D.M. and Garbers, D.L. (2007) Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem. 282:721-730.  PMID: 17098736
“Discovery of a Novel In Vitro Spermatogenesis-Stimulating Factor”

Hamra, F.K., Chapman, K.M., Nguyen, D.M., Williams-Stephens, A.A., Hammer, R.E. and Garbers, D.L. (2005)  Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture. Proc. Natl. Acad. Sci. USA 102:17430-17435.  PMID: 16293688.
“Novel Methods to Derive Proliferating Lines of Fully Functional Rat Sperm Stem Cells”

Hamra, F.K., Schultz, N., Chapman, K.M., Grellhesl, D.M., Cronkhite, J.T., Hammer, R.E. and Garbers, D.L. (2004)  Defining the spermatogonial stem cell. Dev. Biol., 269(2):393-410.  PMID: 15110708
“First Microarray Analysis on Sperm Stem Cells Initiating Spermatogenesis in Culture”

Schultz, N., Hamra, F.K. and Garbers, D.L. (2003)  A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets. Proc. Natl. Acad. Sci. USA 100:12201-12206.  PMID: 14526100
“First Microarray Analysis on Postnatal Development of Sperm Stem Cells in the Mouse”

Hamra, F.K., Gatlin, J., Chapman, K.M., Grellhesl, D.M., Garcia, J.V., Hammer, R.E. and Garbers, D.L.  (2002)  Production of transgenic rats by lentiviral transduction of male germ-line stem cells. Proc. Natl. Acad. Sci. USA 99(23):14931-14936.  PMID: 12391306
“First Report on Transgenic Rats Produced using Germline Stem Cells from Culture”