Instructions for Outside Customers

1. Service Fees

2. Using On-line System

3. Shipping and Handling

1. Service Fees

For users outside of UTSW:

Coomassie Blue	Silver / Sypro Ruby		In solution
$216/sample	$252/sample#1	$420/sample#2	$216/sample	$432/sample#3	$600/sample#3

#1. Sample will be operated at the sensitivity of 100 attomole for standard peptide that gives sufficient sensitivity for the identification of 100 fmol proteins in gel.

#2. Sample will be operated at the sensitivity of 5 attomole for standard peptide that gives sufficient sensitivity for the identification of 20 fmol proteins in gel.

#3. In-solution sample with over 20 proteins identified is charged $432/sample and in-solution sample with over 100 proteins identified is charged $600/sample.

#4. Batch-wise sample submission discount table:

First 9 samples	10th – 49th samples	50th and above samples
Normal price	10% discount	20% discount

#5. LC/MS/MS data will be searched with Mascot software (Matrix Science) and protein identification with Mascot score >=150 is considered as positive result and will be automatically reported. Protein identification with Mascot score less than 150 requires additional manual verification to evaluate the result. Manual verification service is charged at $60/sample.

#6. Turnover time for coomassie blue and in-solution samples is typically 1 business week and turnover time for silver and Sypro Ruby stained samples is typically 2 business weeks.

2. Using On-line System

Sample submission and result retrieval will go through our on-line system. First time users need to sign up an account, then click "Submit Samples" button on the top and follow the link to submit samples. Please also read through the instructions to obtain any detail information.

When the results are ready, a notification email will be sent to you. Please log in first and then click "Retrieve Results" button on the top and follow the link to retrieve the results. A window will pop-up to display the results so please disable any pop-up blocker program temporarily.

3. Shipping and Handling

Extra cauntion must be used to prevent keratin contamination to the gel. Every item that directly contacts with gel slices should be cleaned thoroughly. Please read the instruction for keratin prevention.

If you prefer that we cut the bands, please wrap the whole gel in a plastic film and ship the gel and gel image copy to us using overnight shipping. Leak proof shipping refrigerant should be used. No dry ice.

If you cut the gel slices by yourself, please put each sample in a new eppendorf tube and ship them with gel image copy to us using overnight shipping. Leak proof shipping refrigerant should also be used. No dry ice.

If you send us protein samples in solution, please ship them on dry ice.

Our shipping address is:

Protein ID Lab, UT Southwestern Medical Center
6001 Forest Park Road Room# ND6.202
Dallas, TX 75390-8816
Telephone: 214-645-6301     Fax: 214-645-6298