Welcome to the Mirzaei lab in the Department of Biochemistry at UT Southwestern Medical Center in Dallas, Texas. We develop and use state-of-the-art mass-spectrometry proteomics techniques to address important biological questions.
Research in the lab is centred around developing proteomics methods that can be applied to a wide range of important biological research. We have interests including: the in-depth study of protein ubiquitination; comprehensive identification of protein complexes; identification of drug and pathogen targets; development of methods and software to improve proteomics data analysis.
Take a look at the research page for more details.
Our newest work, published in Mol. Cell. Proteomics is Confetti, a multi-enzyme map of the HeLa proteome. We used 48 single and combination digests to exhaustively analyze HeLa lysate using Orbitrap Elite and Q Exactive mass-spectrometry. We provide an online resource for researchers to identify the best digest to achieve coverage of a protein or region of sequence of interest. In addition, we demonstrated that alternative enzyme digest may give better sensitivity for some proteins in SRM experiment.
See the paper at the link below:
Confetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics.
Guo X*, Trudgian DC*, Lemoff A, Yadavalli S, Mirzaei H Mol. Cell Proteomics 2014 Apr
* Equal Contribution
Access our Confetti map at proteomics.swmed.edu/confetti
Take a look at our full list of publications.
Lead by Dr. Mirzaei, the lab consists of faculty, postdocs and staff with considerable expertise in mass-spectrometry, bioinformatics, biochemistry & cell biology. Visit our people page for photos, bios and contact info.