Selected Reaction Monitoring (SRM) is an emerging proteomics technology with the power to transform proteomics into an effective data acquisition platform with all the requirements posed by the systems biology roadmap. Recognizing its potential, in the past few years we have been focusing on the development and application of SRM mass spectrometry. Building upon this knowledge, my lab's research program will be focused on the development of analytical technologies that would alleviate limitations currently hindering proteomics from becoming an accurate high throughput data acquisition technology. In this effort we are focused on developing enhanced high resolution, high capacity separation and detection platform based on emerging SRM mass spectrometry for improved protein detection and quantification. My lab is also focused on developing new bioinformatics tools for processing the data generated by these advanced mass spectrometry methods. In the biological front we are focused on developing SRM assays to study transcription regulation and protein ubiquitination. In this effort a portion of my lab's research program is dedicated to the development of new enrichment techniques for class-specific and post-translationally modified proteins, a step essential for successful analyses of low abundance proteins.
My lab's research program encompasses innovation of protein enrichment methods, advances in SRM mass spectrometry techniques, and development of problem-specific computational tools for data processing. This program will serve as a solid foundation for large-scale integrative experiments that will provide the accurate and comprehensive data needed to gain insight into the complex cellular regulatory mechanisms.