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Nguyen, A.D., Lee, S.H., DeBose-Boyd, R.A. (2009) Insig-mediated, sterol-accelerated degradation of the membrane domain of hamster 3-hydroxy-3-methylglutaryl-coenzyme A reductase in insect cells.
J. Biol. Chem. 284: 26778-88.
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Lee, P.C., Debose-Boyd, R.A. (2009) Intramembrane glycine mediates multimerization of Insig-2, a requirement for sterol regulation in Chinese hamster ovary cells.
J. Lipid Res. Jul 17. [Epub ahead of print]
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Debose-Boyd, R.A. (2008) Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase.
Cell Res. 18: 609-621.
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Cao, J., Wang, J., Qi, W., Miao, H.H., Wang, J., Ge, L., Debose-Boyd, R.A., Tang, J.J., Li, B.L., and Song, B.L. (2007) Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by
regulating the stability of HMG-CoA reductase. Cell Metab. 6: 115-128.
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Nguyen, A.D., McDonald, J.G., Bruick, R.K., and Debose-Boyd, R.A. (2007) Hypoxia stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A
reductase through accumulation of lanosterol and hypoxia-inducible
factor-mediated induction of insigs. J. Biol. Chem. 282: 27436-27446.
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Lee, P.C., Liu, P., and Debose-Boyd, R.A. (2007) Amplification of the gene for SCAP, coupled with Insig-1 deficiency, confers sterol resistance in mutant Chinese hamster ovary cells. J. Lipid. Res. 48, 1944-1954.
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Debose-Boyd, R.A. (2007) A helping hand for cytochrome p450 enzymes. Cell Metab. 5: 81-83.
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Lee, P.C., Nguyen, A.D., and DeBose-Boyd, R.A. (2007) Mutations within membrane domains of HMG CoA reductase confer resistance to sterol-accelerated degradation. J. Lipid Res. 48, 318-327.
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Lee, J.N., Song, B., and Debose-Boyd, R.A. (2006) Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin
ligase, gp78. J. Biol. Chem. 281, 39308-39315.
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Song, B.L., and DeBose-Boyd, R.A. (2006) Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl
coenzyme a reductase stimulated by delta- and gamma-tocotrienols. J. Biol. Chem. 281, 25054-25061.
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Goldstein, J.L, DeBose-Boyd, R.A., and Brown, M.S. (2006) Protein sensors for membrane sterols.
Cell, 124, 35-46.
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Song, B.L., Sever, N., and DeBose-Boyd, R.A. (2005) Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples
sterol-regulated ubiquitination to degradation of HMG CoA reductase. Mol. Cell 19, 829-840.
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Song, B.L., Javitt, N.B., and DeBose-Boyd,
R.A. (2005) Insig-mediated degradation of HMG CoA reductase
stimulated by lanosterol, an intermediate in the synthesis
of cholesterol. Cell Metab 1, 179-189.
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Lee, P.C., Sever, N., and DeBose-Boyd, R.A. (2005) Isolation of sterol-resistant Chinese hamster ovary cells with genetic
deficiencies in both Insig-1 and Insig-2. J. Biol. Chem. 280, 25242-25249.
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DeBose-Boyd, R.A. (2004) Knowing
when to let go: endosomal release of LDL from the LDL-receptor.
Mol. Cell 16, 160-162
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Sever, N., Lee, P.C.W, Song, B.-L., Rawson,
R.B., and DeBose-Boyd, R.A. (2004) Isolation of mutant
cells lacking Insig-1 through selection with SR-12813,
an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl
coenzyme A reductase. J. Biol. Chem. 279, 43136-43147.
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Sever, N., Song, B.-L., Yabe, D., Goldstein,
J.L., Brown, M.S., and DeBose-Boyd, R.A. (2003) Insig-Dependent
Ubiquitination and Degradation of Mammalian 3-Hydroxy-3-methylglutaryl
CoA Reductase Stimulated by Sterols and Geranylgeraniol.
J. Biol. Chem. 278, 52479-52490.
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SIGNIFICANT PUBLICATIONS
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Song, B.-L. and DeBose-Boyd, R.A. (2004) Ubiquitination
of 3-hydroxy-3-methylglutaryl CoA reductase in permeabilized
cells mediated by cytosolic E1 and a putative membrane-bound
ubiquitin ligase. J. Biol. Chem. 279, 28798-28806.
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Sever, N., Yang, T., Brown,
M.S., Goldstein, J.L., and DeBose-Boyd, R.A. (2003)
Accelerated Degradation of HMG CoA Reductase Mediated
by Binding of Insig-1 to its Sterol-Sensing Domain.
Mol. Cell 11, 25-33. |
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DeBose-Boyd, R.A., Brown, M.S.,
Li, W.-P., Nohturfft, A., Goldstein, J.L., and Espenshade,
P.J. (1999) Transport-dependent proteolysis of SREBP:
Relocation of Site-1 Protease from Golgi to ER obviates
the need for SREBP transport to Golgi. Cell 99, 703-712.
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