Coverage includes techniques for isolating, analyzing and cloning both large and small DNA molecules; cDNA cloning
and exon trapping, amplification of DNA, generation and use of nucleic acids probes, mutagenesis, and DNA sequencing;
and methods to screen expression libraries, express cloned genes in both pro- and eukaryotic cells, analyze transcripts
and proteins, and detect protein-protein interactions. In addition to annotations within protocols, 115 information panels
spread throughout the volumes provide insight into the reasons why methods are carried out in a certain manner and how
techniques have evolved. The appendices are a compendium of reagents, vectors, media, techniques, suppliers, kits, and electronic resources.
Updated text includes revised protocols and new material for students in genetics, molecular cell biology, developmental biology,
microbiology, neuroscience, and immunology. Describes techniques, cDNA cloning and more. Softcover, hardcover also available.
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled
reputation for reliability, accuracy, and clarity.
In this new edition, authors Joe Sambrook and David Russell have completely updated the
book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for
studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential
for everyone using today's biomolecular techniques.
The opening chapters describe essential techniques, some well-established, some new, that are used every day
in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of
nucleic acid probes, mutagenesis, and DNA sequencing.
The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes
and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.
The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.
As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information
about why techniques work, how they were first developed, and how they have evolved.
Paperback: 999 pages; Dimensions (in inches): 5.45 x 11.65 x 9.35
*Publisher: Cold Spring Harbor Laboratory; 3rd edition (January 15, 2001)
*Other Editions: Hardcover | Library Binding | Spiral-bound (2nd Edition/3 Volume Set)
Molecular Cloning: A Laboratory Manual (3-Volume Set) by Joseph Sambrook, David W. Russell