Core Request Form
Primary Investigator *

Request Special Instructions

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Institution *
Address *
City *
State *
Phone *
E-mail Address *
Funding Agency *
Kidney Related Grant Title & Number *
Lab Contact *
Phone *
Email*
Animal Core Services

Generation and distribution of transgenic mice expressing kidney specific tetracycline-inducible activator and repressor.
Assistance in the generation of additional transgenic mice and knockout and knockin mice.
Training investigators to perform embryonic kidney organ cultures.
Distribution of transgenic Mice--Ksp/Cre (Please Specify Gene & Brief Research Plan in Special Instructions)**
Distribution of transgenic Mice---Ksp.CreERT2 (Please Specify Gene & Brief Research Plan in Special Instructions)**
Distribution of transgenic Mice---Pkhd1/Cre (Please Specify Gene & Brief Research Plan in Special Instructions)**
Performing and training investigators in the use of kidney injury/repair models including renal ischemia-reperfusion injury, ureteral obstruction, chemical injury and bone marrow stem cell transplantation

 **Animal Core Special Instructions
**Shipping Coordinator(You must provide if Requesting Animals)**
Name: **Shipping Address for mice:
Phone:
Email:
**Veterinarian(You must provide if Requesting Animals)**
Name:
Phone:
Email:
Physiology Core Services
Measurement of serum Cr in mice by capillary electrophoresis.
Measurement of serum and urine chemistry and blood gas.
Microanalysis of serum and urine electrolytes.
Blood pressure measurement in mice and rats by tail-cuff method. Equipment and training provided to investigators free of charge
Clearance Studies. Core will provide metabolic cages (10 available) and advice to investigators free of charge
Microdissection of tubules for quantitation of proteins/gene expression. (By Collaboration Only)
Microperfusion Studies of Renal Tubules. (By Collaboration Only
Electrophysiological Studies of ion Channels by patch-clamp recordings. (By Collaboration Only)
 Physiology Core Special Instructions
Cell Biology and Pathology Services
Histology (tissue fixation, embedding, and staining)
Consult for immunohistochemistry/lectin and enzyme histochemistry and In Situ hybridization
Light and Confocal Microscopy and Image Analysis
Laser Microdissection
Kidney Pathology
Training in select procedures
Distribution of Plamids		 Cell Biology & Pathology Special Instructions
Clinical and Transitional Services
Human research protocol design, methods, and implementation and conduct
Study participant recruitment and management
Regulatory document preparation and monitoring
Biostatistical support for both human and laboratory studies
Blood and urine sample collection, preparation, storage and transportation
Data collection for phenotyping human research subjects
Database development and management
Ambulatory blood pressure
Specialized analyses of serum and urine analytes including iothalamate
Training in methods of clinical and translational research including protocol development, human research subject, and institutional review board documentation		 Clinical & Translational Special Instructions
We ask that you agree to acknowledge the UT Southwestern OBrien Kidney Core Research Center NIH P30DK079328 on any and all publications. If you agree, please check the following box before this request will be submitted. This will contitute your agreement of acknowledging the UT Southwestern O'Brien Kidney Core Research Center. *
* I agree to acknowledge the UT OBrien Kidney Core Research Center on any publications and/or grant applications and also provide the information to the center.
* Required