Vibrio parahaemolyticus T3SS2
The second pathogenicity island of Vibrio parahaemolyticus contains T3SS2 and is associated with enterotoxicity. We have identified the activity of two effectors on this island. The first, VopA, is a homologue of Yersinia YopJ and also displays acetylatransferase activity (Trosky et al., J Biol Chem 2007). Another effector, VopL, during infection promotes the assembly of actin stress fibers and in vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors (Liverman et al., PNAS USA 2007).
Publications on T3SS2
- Santos, M.S.. & Orth, K. Intracellular Vibrio parahaemolyticus Escapes the Vacuole and Establishes a Replicative Niche in the Cytosol of Epithelial Cells. (2014) mBio (In Press).
- Calder, T., Kinch, L.N., Fernandez, J., Salomon, D., Grishin, N.V., & Orth, K. Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxin PLoS ONE (In Press).
- Ham, H. & Orth, K. The Role of Type III Secretion System 2 in Vibrio parahaemolyticus Pathogenicity. J Microbiol. (2012) Oct;50(5):719-25. PMID:23124738[PubMed - in process]
- Zhang, L.L, Krachler, A-M, Broberg, CA, Li, Y, Mirzaei, H, Gilpin, CJ & Orth, K. Type III effector VopC mediates invasion for Vibrio species. Cell Reports (2012) 1(5): 453-460.
- Trosky, J. E., Li, Y., Mukherjee, S., Keitany, G., Ball, H. and Orth, K. VopA Inhibits ATP binding by Acetylating the Catalytic Loop of MKKs. (2007) J. Biol. Chem. 282: 34299-34305.
Liverman, A.D.B., Cheng, H-C., Trosky, J., Leung, D.W., Melanie L. Yarbrough, Dara L. Burdette, Michael K. Rosen & Orth, K. Direct Assembly of Actin by Vibrio Type III Effector VopL. (2007) PNAS USA 104: 17117-17122.
Trosky, J., Mukherjee, S., Burdette, D., Roberts, M., McCarter, L., Siegel, R. & Orth, K. Inhibition of MAPK signaling pathways by VopA from Vibrio parahaemolyticus. (2004) J. Biol. Chem., 279, 51953-51957.