Research
YopJ and Ser/Thr Acetylation
Studies in my lab focused on YopJ, a bacterial effector expressed by Yersinia. This pathogen, the causal agent of the Plague, uses YopJ to shut down the signaling machinery responsible for eliciting an immune response. We discovered that YopJ uses a novel post-translational modification, Ser/Thr acetylation to modify kinases and prevent their activation by phosphorylation (Science. 323:269-272, 2009).
Publications on AMPylation
- Hao, Y-H., Wang, Y., Burdette, D., Mukherjee, S., Keitany, G., Goldsmith, E., and Orth, K. Structural requirements for Yersinia YopJ inhibition of MAP kinase pathways. (2008) PLoS ONE 1: 1-9 e1375.
- Mukherjee, S. and Orth, K. In vitro signaling by MAPK and NFkB pathways inhibited by Yersinia YopJ. (2008) Methods in Enzymology 438: 343-353.
- Mukherjee, S., Hao, Y-H., and Orth, K. New posttranslational modification on Ser/Thr residues by acetylation (2007) Trends in Biochemical Sciences 32: 210-216.
- Chosed, R., Tomchick, D.R., Brautigam, C.A., Machius, M.2, & Orth, K. Structural analysis of Xanthomonas XopD provides insights into substrate specificity of Ulp1s. (2007) J. Biol. Chem. 282:6773-6782.
- Trosky, J. E., Li, Y., Mukherjee, S., Keitany, G., Ball, H. and Orth, K. VopA Inhibits ATP binding by Acetylating the Catalytic Loop of MKKs. (2007) J. Biol. Chem. 282: 34299-34305.
- Chosed, R., Mukherjee, S. & Orth, K. Impact of regulatory domains on activity and specificity of Arabidopsis thaliana Ulp1s. (2006) Biochemical J., 398, 521-529.
- Mukherjee, S. Kietany, G., Li, Y Wang, Y., Hall B.E., Goldsmith, E & Orth, K. Yersinia YopJ Acetylates and Inhibits Kinase Activation by Preventing Phosphorylation. (2006) Science, 312:1211-1214.
- Yoon, S., Lui, Z., Eyobo, Y. & Orth, K. Yersinia Effector YopJ Inhibits Yeast MAPK signaling pathways by an evolutionarily conserved mechanism. (2003) J. Biol. Chem., 278, 131-2135.
- Orth, K., Xu, Z., Mudgett, M.B., Bao, Z.Q., Mangel, W.F., Staskawicz, B. & Dixon, J.E. Yersinia effector YopJ is a Ubiquitin-like protein protease that disrupts signaling. (2000) Science, 290, 1594-1597.
- Orth, K., Palmer, L.P., Zhao Qin Bao, Z.Q.,Stewart, S., Rudolph, A., Bliska, J.B. & Dixon, J.E. A Yersinia Virulence Factor Inhibits Activation of the Mitogen Activated Protein Kinase Kinase Superfamily. (1999) Science, 285, 1920-1923.