Software Downloads
Two software programs, tcaSIM and
tcaCALC detailed below, are available for
download. To download the software, go to Registration and Download and complete
the registration form. If for some reason this should
fail, please contact Mark Jeffrey.
tcaSIM
The tcaSIM program is a tool for use with
labeling studies in intermediary metabolism - primarily those
applying 13C NMR spectroscopy, but also for
14C radiolabel studies and
13C mass isotopomer analysis. The
simulation has been developed to assist in the design of
experiments and to help the user understand the effect of various
metabolic states and substrate enrichment levels on labeling
patterns.
tcaCALC
The tcaCALC program performs an isotopomer
analysis to estimate relative pathway fluxes in biological
systems at metabolic and isotopic steady state. It is used
in 13C labeling studies where biological
systems (isolated cells, perfused organs or intact animals) are
provided enriched substrate(s), and the labeling of intermediates
detected using 13C NMR spectroscopy, mass-
and tandem mass spectrometry. The 13C
spectra are collected either directly from intact tissue, such as
in the case of perfusions conducted inside a magnet, or after
extraction of a tissue sample (isolated cell studies or tissue
samples taken after in vivo infusion of
13C-enriched substrates).
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