Protocols

Cellular Biology Protocols

• Histology Protocol
• Soft Agar Assays
• Uptake of AlexaFluor 633-Transferrin by Flow Cytometry
• Monoclonal Antibody Overview
• Polyclonal Antibody Overview

Constructs and Probes

• Screening Strategy for conditional HIP1 knock-out
• Creating a Plasmid in Vector NTI 5
• Screening Strategy for HIP1/PDGFβR knock-in
• Making 3' Probe for Conditional Knock-Out Mice
• Making 5' Probe for Conditional Knock-Out Mice
• Making 3' Probe for Knock-In Mice
• Making 5' Probe for Knock-In Mice

Mouse Techniques

• Creating mating cages
• Embryonic lethality
• Mouse embryo fibroblast (MEF) protocol
• Mouse colony nomenclature
• Mouse sera analysis
• Overview of PCR analyses
• Protocol for making chimeric mice

Southern Blot Techniques

• Hybridizing Southern Using Random Primed DNA Labeling Kit
• Southern Blot Protocol
• Preparation of genomic DNA
• Probes for Southern blotting
• Reagents and supplies for Southern blots