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Publications

Click here for a complete list of publications

Antibody Engineering and The MHC Class I-Related Receptor, FcRn

• Montoyo, HP., Vaccaro, C., Hafner, M., Ober, R. J., Mueller, W., and Ward, E. S. (2009) Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice, Proc. Natl. Acad. Sci. USA , 106, 2788-2793.
• Gan, Z., Ram, S., Vaccaro, C., Ober, R. J., and Ward, E. S. (2009) Analyses of the recycling receptor, FcRn, in live cells reveal novel pathways for lysosomal delivery, Traffic , 10, 600-614.
• Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J. and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy, Proc. Natl. Acad. Sci. USA , 104, 5889-5894.
• Vaccaro, C., Bawdon, R., Wanjie, S., Ober, R. J. and Ward, E. S. (2006) Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies, Proc. Natl. Acad. Sci. USA , 103, 18709-18714.
• Vaccaro, C., Zhou, J., Ober, R. J. and Ward, E. S. (2005) Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels, Nature Biotech. , 23, 1283-1288.
• Zhou, J., Mateos, F., Ober, R. J. and Ward, E. S. (2005) Conferring the binding properties of the mouse MHC Class I-related receptor, FcRn, onto the human ortholog by sequential rounds of site-directed mutagenesis, J. Mol. Biol. , 345, 1071-1081.
• Ober, R. J., Martinez, C., Lai, X., Zhou, J. and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level, Proc. Natl. Acad. Sci. USA , 101, 11076-11081.
• Ober, R. J., Martinez, C., Vaccaro, C., Zhou, J., and Ward, E. S. (2004) Visualizing the site and dynamics of IgG salvage by the MHC Class I-related receptor, FcRn, J. Immunol. , 172, 2021-2029.
• Zhuo, J., Johnson, J., Ghetie, V., Ober, R. J. and Ward, E. S. (2003) Generation of mutated variants of the human form of the MHC class I-related receptor, FcRn, with increased affinity for mouse immunoglobulin G, J. Mol. Biol. , 332, 901-913.
• Kim, J-K., Firan, M., Radu, C., Kim, C-H., Ghetie, V. and Ward, E. S. (1999) Mapping of the site on human IgG1 for binding of the MHC class I related receptor, FcRn, Eur. J. Immunol. , 29, 2819-2825.
• Ghetie, V., Popov, S., Borvak, J., Radu, C., Matesoi, D., Medesan, C., Ober, R. J. and Ward, E. S. (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis, Nature Biotech. , 15, 637-640.
• Medesan, C., Matesoi, D., Radu, C., Ghetie, V. and Ward, E. S. (1997) Delineation of the amino acid residues involved in transcytosis and catabolism of mouse IgG, J. Immunol. , 158, 2211-2217.
• Ghetie, V., Hubbard, J. G., Kim, J-K., Tsen, M-F., Lee, Y. and Ward, E. S. (1996) Abnormally short serum half lives of IgGs in beta2-microglobulin deficient mice, Eur. J. Immunol. , 26, 690-696.
• Ward, E.S., Güssow, D., Griffiths, A., Jones, P.T. and Winter, G. (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli, Nature , 341, 544-546.

Autoimmune Disease and T cell Biology

• Pastor, S., Minguela, A., Mi, W., and Ward, E. S. (2009) Autoantigen immunization at different sites reveals a role for anti-inflammatory effects of IFN-gamma in regulating susceptibility to experimental autoimmune encephalomyelitis , J. Immunol. , 182, 5268-5275.
• Mi, W., Wanjie, S., Lo, S. T., Gan, Z., Pickl-Herk, B., Ober, R. J., and Ward, E. S. (2008) Targeting the neonatal Fc receptor for antigen delivery using engineered Fc fragments , J. Immunol. , 181, 7550-7561.
• Minguela, A., Pastor, S., Mi, W., Richardson, J. A., and Ward, E. S. (2007) Feedback regulation of murine autoimmunity via dominant anti-inflammatory effects of interferon gamma , J. Immunol. , 178, 134-144.
• Huang, J.C., Ober, R. J. and Ward, E. S. (2005) The central residues of a T cell receptor sequence motif are key determinants of autoantigen recognition in murine experimental autoimmune encephalomyelitis , Eur. J. Immunol. , 35, 299-304.
• Huang, J. C., Han, M., Minguela, A., Pastor, S., Qadri, A. and Ward, E.S. (2003) T cell recognition of distinct peptide: I-Au conformers in murine experimental autoimmune encephalomyelitis , J. Immunol. , 171, 2467-2477.
• Machius, M., Cianga, P., Deisenhofer, J. and Ward, E. S. (2001) Crystal structure of a T cell receptor Va11 (AV11S5) domain: new canonical forms for the first and second complementarity determining regions , J. Mol. Biol. , 310, 689-698.
• Garcia, K. C., Radu, C. G., Ho, J., Ober, R.J. and Ward, E. S. (2001) Kinetics and thermodynamics of T cell receptor-autoantigen interactions in murine experimental autoimmune encephalomyelitis , Proc. Natl. Acad. Sci. USA , 98, 6818-6823.
• Qadri, A., Radu, C. G., Thatte, J., Cianga, P., Ober, B. T., Ober, R. J. and Ward, E. S. (2000) A role for the region encompassing the c” strand of a T cell receptor Va domain in T cell activation events , J. Immunol. , 165, 820-829.
• Thatte, J., Qadri, A., Radu, C. and Ward, E. S. (1999) Molecular requirements for T cell recognition by an MHC Class II restricted T cell receptor: the involvement of the fourth hypervariable loop of the Va domain , J. Exp. Med. , 189, 509-519.
• Fields, B. A., Ober, B., Malchiodi, E. L., Lebedeva, M. I., Braden, B., Ysern, X., Kim, J-K., Shao, X., Ward, E.S. and Mariuzza, R. A. (1995) Crystal structure of the Va domain of a T cell antigen receptor , Science , 270, 1821-1824.

Fluorescence Microscopy

• Ram, S., Prabhat, P., Ward, E. S., and Ober, R. J. (2009) Improved single particle localization accuracy with dual objective multifocal plane microscopy , Optics Express , 17, 6881-6898.
• Chao, J., Ram, S., Abraham, A., Ward, E. S., and Ober, R. J. (2009) A resolution measure for three-dimensional microscopy , Optics Com. , 282, 1751-1761.
• Gan, Z., Ram, S., Vaccaro, C., Ober, R. J., and Ward, E. S. (2009) Analyses of the recycling receptor, FcRn, in live cells reveal novel pathways for lysosomal delivery , Traffic , 10, 600-614.
• Ram, S., Prabhat, P., Chao, J., Ward, E. S., and Ober, R. J. (2008) High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells , Biophys. J. , 95, 6025-6043.
• Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J. and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy, , Proc. Natl. Acad. Sci. USA , 104, 5889-5894.
• Prabhat, P., Ram, S., Ward, E. S. and Ober, R. J. (2006) Simultaneous imaging of several focal planes in fluorescence microscopy for the study of cellular dynamics in 3D , Proc. SPIE. , 6090, 115-121.
• Ram, S., Ward, E. S., and Ober, R. J. (2006) Beyond Rayleigh’s criterion: A resolution measure with application to single-molecule microscopy , Proc. Natl. Acad. Sci. USA , 103, 4457-4462.
• Ram, S., Ward, E. S., and Ober, R. J. (2006) A stochastic analysis of performance limits for optical microscopes , Multidim. Sys. Sig. Proc. , 15, 27-57.
• Lai, X., Lin Z., Ward, E. S. and Ober R. J. (2005) Noise suppression of point spread functions and its influence on deconvolution of three dimensional fluorescence microscopy image sets , J. Microscopy , 217, 93-108.
• Ober, R. J., Lai, X., Lin Z. and Ward E. S. (2005) State Space Realization of a three-dimensional image set with application to noise reduction of fluorescent microscopy images of cells, , Multidim. Sys. Sig. Proc. , 16, 7-47.
• Ober, R. J., Martinez, C., Lai, X., Zhou, J., and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level , Proc. Natl. Acad. Sci. USA , 101, 11076-11081.
• Prabhat, P., Ram, S., Ward, E. S. and Ober, R. J. (2004) Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions. , IEEE Trans. Nanobio. , 3, 237-242.
• Ober, R. J., Ram, S. and Ward, E. S. (2004) Localization accuracy in single-molecule microscopy , Biophys. J. , 86, 1185-1200. Please see MIATool for more information.

Signal Processing

• Zou, Q., Lin, Z., Ober, R. J. (2006) The Cramer Rao lower bound for bilinear systems , IEEE Trans. Sig. Proc. , 54, 1666-1680.
• Lin, Z., Zou, Q., Ward, E. S. and Ober, R. J. (2005) The Cramer Rao lower bound for parameter estimation in nonlinear systems , IEEE Sig. Proc. Letts. , 12, 855-858.
• Ober, R. J., Lai, X., Lin, Z. and Ward, E. S. (2005) State space realization of a three-dimensional image set with application to noise reduction in fluorescent microscopy images of cells , Multidim. Sys. Sig. Proc. , 16, 7-48.
• L. R. Hunt, E. Sally Ward, and Raimund J. Ober. (2004) Approximation of trajectories of nonlinear systems by iterates of systems with linear state dynamics , Sys. Cont. Letts. , 51, 377-381.
• Ober, R. J.,Lin, Z., and Zou, Q. (2003) Calculations of the Fisher information matrix for multidimensional data sets , IEEE Trans. on Sig. Proc. , 51(10), 2679–2691.
• Ober, R. J. , Lin, Z., Ye, H. and Ward, E. S. (2002) Achievable accuracy of parameter estimation for multidimensional NMR experiments , J. Mag. Reson. , 157, 1-16.
• Ober, R. J. (2002) The Fisher information matrix for linear systems , Sys. Contr. Letts. , 47, 221–226.

Surface Plasmon Resonance (SPR)

• Ober, R. J., Caves, J. and Ward, E. S. (2003) Analysis of exponential data using a non-iterative technique: application to surface plasmon experiments , Anal. Biochem. , 312, 57-65.
• Ober, R. J. and Ward, E. S. (2002) Compensation for loss of ligand activity in surface plasmon resonance experiments , Anal. Biochem. , 306, 228-236.
• Ober, R. J. and Ward, E. S. (1999) The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments. , Anal. Biochem. , 271, 70-80. Please see SPRTool for more information.