The Wülfing laboratory is interested in understanding how the spatiotemporal organization of lymphocyte signaling systems regulates lymphocyte function.
To determine the spatiotemporal organization of lymphocyte signaling, we utilize live cell fluorescence microscopy and develop quantitative image analysis methodology. An overview of our determination of the spatiotemporal organization of T cell activation with > 50 elements analyzed is given in the section ‘Spatiotemporal patterning of T cell activation’. We are continually expanding our data set and gladly image additional sensors in collaboration with outside investigators (please contact us if interested).
To determine how the spatiotemporal organization of lymphocyte signaling regulates function, we have used various approaches to alter the localization of key signaling intermediates and determined the functional consequences. These findings are contained in our most recent publications.
