Publications

1999 to Present


Peer-reviewed Publications


  1. Luo , X., Fang, G., Coldiron , M., Lin, Y., Yu, H.*, Kirschner , M. W. and Wagner, G.*(2000) Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Nat. Struct . Biol. 7 , 224-229 (*Corresponding authors) (PDF)
  2. Tang, Z., Bharadwaj , R., Li, B. and Yu, H. (2001) Mad2-independent inhibition of APC Cdc20 by the mitotic checkpoint protein BubR1. Dev. Cell 1 , 227-237. (PDF)
  3. Tang, Z., Li, B., Bharadwaj , R., Zhu, H., Özkan , E., Hakala , K., Deisenhofer, J. and Yu, H. (2001) APC2 cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell 12 , 3839-3851. (PDF)
  4. Bembenek , J. and Yu, H. (2001) Regulation of the anaphase-promoting complex by the human dual-specificity phosphatase hCdc14a. J. Biol. Chem. 276 , 48237-48242. (PDF)
  5. Luo , X., Tang, Z., Rizo , J. and Yu, H. (2002) The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20. Mol. Cell 9 , 59-71. (PDF)
  6. He, Q., Cheng P., Yang, Y., He, Q., Yu, H. and Liu, Y. (2003) Fbw1-mediated degradation of FREQUENCY in Neurospora establishes a conserved mechanism for circadian clock regulation. EMBO J., 22 , 4421-4430. (PDF)
  7. Agarwal , R., Tang, Z., Yu, H., and Cohen-Fix, O. (2003) Two distinct pathways for inhibiting Pds1 ubiquitination in response to DNA damage. J. Biol. Chem., 278, 45027-45033. (PDF)
  8. Araki, M., Wharton, R. P., Tang, Z., Yu, H., and Asano, M. (2003) Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. EMBO J., 22 , 6115-6126. (PDF)
  9. Luo , X., Tang, Z., Xia, G., Wassmann , K., Matsumoto, T., Rizo , J., and Yu, H. (2004) The Mad2 spindle checkpoint protein has two distinct natively folded states. Nat. Struct . Mol. Biol., 11 , 338-345. (PDF)
  10. Bharadwaj , R., Qi , W., and Yu, H. (2004) Identification of two novel components of the human Ndc80 kinetochore complex. J. Biol. Chem., 279 , 13076-13085. (PDF)
  11. Wang, W.-Q., Bembenek , J., Gee, K. R., Yu, H., Charbonneau, H., Zhang, Z.-Y. (2004) Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14. J. Biol. Chem., 279 , 30459-30468. (PDF)
  12. Xia, G., Luo , X., Habu , T., Rizo , J., Matsumoto, T., and Yu, H. (2004) Conformation-specific binding of p31 comet antagonizes the function of Mad2 in the spindle checkpoint. EMBO J., 23 , 3133-3143. (PDF)
  13. Tang, Z., Shu , H., Oncel , D., Chen, S., and Yu, H. (2004) Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell, 16 , 387-397. (PDF)
  14. Wang, R.-H., Yu, H. and Deng, C.-X. (2004) A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint. Proc. Natl. Acad. Sci . U. S. A., 101 , 17108-17113. (PDF)
  15. Tang, Z., Sun, Y., Harley, S. E., Zou , H., and Yu, H. (2004) Human Bub1 protects centromeric sister- chromatid cohesion through Shugoshin during mitosis . Proc. Natl. Acad. Sci . U. S. A., 101 , 18012-18017. (PDF)
  16. Liu, B., Hong, S., Tang, Z., Yu, H., and Giam , C.-Z. (2005) HTLV-I Tax directly binds the Cdc20-associated anaphase-promoting complex and activates it ahead of schedule. Proc. Natl. Acad. Sci . U. S. A., 102 , 63-68. (PDF)
  17. Gocke , C. B., Yu, H.*, and Kang, J. (2005) Systematic identification and analysis of mammalian SUMO substrates. J. Biol. Chem., 280 , 5004-5012. (*Corresponding author) (PDF)
  18. Bembenek , J., Kang, J., Kurischko , C., Li, B., Raab , R. R., Belanger, K. D., Luca, F. C., and Yu, H. (2005) Crm1-dependent nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast. Cell Cycle, 4 , 961-971. (PDF)
  19. Potts, P. R. and Yu, H. (2005) Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol., 25 , 7021-7032. (PDF)
  20. Araki, M., Yu, H., and Asano, M. (2005) A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo . Genes Dev., 19 , 2458-2465. (PDF)
  21. Özkan , E., Yu, H.*, and Deisenhofer, J.* (2005) Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases . Proc. Natl. Acad. Sci . U. S. A., 102 , 18890-18895. (*Corresponding authors) (PDF)
  22. Kang, J., Gocke , C. B., and Yu, H. (2006) Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity. BMC Biochemistry, 7 , 5. (PDF)
  23. Tang, Z., Shu , H., Qi , W., Mahmood , N., Mumby, M. C., and Yu, H. (2006) PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation. Dev. Cell, 10 , 575-585. (PDF)
  24. Potts, P. R., Porteus , M. H., and Yu, H. (2006) Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks. EMBO J., 25 , 3367-3376. (PDF)
  25. Qi , W., Tang, Z., and Yu, H. (2006) Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1. Mol. Biol. Cell, 17 , 3705-3716. (PDF)
  26. Yang, M., Gocke , C. B., Luo , X., Borek , D., Tomchick , D. R., Machius , M., Otwinowski , Z., and Yu, H. (2006) Structural basis for CoREST -dependent demethylation of nucleosomes by the human LSD1 histone demethylase . Mol. Cell, 23 , 377-387. (PDF)
  27. Liu, C.-W., Li, X. Thompson, D., Wooding, K., Chang, T.-L., Tang, Z., Yu, H., Thomas, P. J., and DeMartino , G. N. (2006) ATP binding and ATP hydrolysis play distinct roles in the function of 26S proteasome. Mol. Cell, 24 , 39-50. (PDF)
  28. Kang, Y. H., Park, J.-E., Yu, L.-R., Soung , N.-K., Yun , S.-M., Bang, J. K., Seong , Y.-S., Yu, H., Garfield, S., Veenstra , T. D., and Lee, K. S. (2006) Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation. Mol. Cell, 24 , 409-422. (PDF)
  29. Qi , W. and Yu, H. (2007) KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome. J. Biol. Chem., 282 , 3672-3679. (PDF)
  30. Yang, M., Culhane , J. C., Szewczuk , L. M., Gocke , C. B., Brautigam , C. A., Tomchick , D. R., Machius , M., Cole, P. A., and Yu, H. (2007) Structural basis of histone demethylation by LSD1 revealed by suicide inactivation. Nat. Struct . Mol. Biol., 14 , 535-539. (PDF)
  31. Szewczuk , L. M., Culhane , J. C., Yang, M., Majumdar , A., Yu, H., and Cole, P. A. (2007) Mechanistic analysis of a suicide inactivator of histone demethylase LSD1. Biochemistry, 46 , 6892-6902. (PDF)
  32. Potts, P. R. and Yu, H. (2007) The SMC5/6 complex maintains telomere length in ALT cancer cells through sumoylation of telomere-binding proteins. Nat. Struct . Mol. Biol., 14 , 581-590. (PDF)
  33. Yang, M., Culhane , J. C., Szewczuk , L. M., Jalili , P., Ball, H. L., Machius , M., Cole, P. A., and Yu, H. (2007) Structural basis for inhibition of the LSD1 histone demethylase by the antidepressant trans-2-phenylcyclopropylamine. Biochemistry, 46 , 8058-8065. (PDF)
  34. Yang, M., Li, B., Tomchick , D. R., Machius , M., Rizo , J., Yu, H., and Luo , X. (2007) p31 comet blocks Mad2 activation through structural mimicry. Cell, 131 , 744-755. (PDF)
  35. Kang, J., Chen, Y., Zhao, Y., and Yu, H. (2007) Autophosphorylation -dependent activation of human Mps1 is required for the spindle checkpoint. Proc. Natl. Acad. Sci . U. S. A., 104 , 20232-20237. (PDF)
  36. Yang, M., Li, B., Liu, C.-J., Tomchick , D. R., Machius , M., Rizo , J., Yu, H.*, and Luo , X*. (2008) Insights into Mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric Mad2 dimer. PLoS Biol., 6 , e50.   (*Corresponding authors) (PDF)

Invited Reviews, Commentaries, and Book Chapters:


  1. Yu, H. (1999) Extending the size limit of protein nuclear magnetic resonance. Proc. Natl. Acad. Sci . U. S. A. 96 , 332-334. (PDF)
  2. Fang G., Yu, H., and Kirschner M. W. (1999) Control of mitotic transitions by the anaphase-promoting complex. Philos . Trans. R. Soc. Lond . B Biol. Sci . 354 , 1583-1590.
  3. Yu, H. (2002) Regulation of APC–Cdc20 by the spindle checkpoint. Curr . Opin . Cell Biol., 14 , 706-714. (PDF)
  4. Bembenek , J. and Yu. H. (2003) Regulation of Cdc14: pathways and checkpoints of mitotic exit. Frontiers Biosci ., 8 , 1275-1287. (PDF)
  5. Bharadwaj , R. and Yu, H. (2004) The spindle checkpoint, aneuploidy , and cancer. Oncogene, 23 , 2016-2027. (PDF)
  6. Tang, Z. and Yu, H. (2004) Functional analysis of the spindle checkpoint proteins using an in vitro ubiquitination assay. Methods Mol. Biol., 281 , 227-242.
  7. Yu, H. and Tang, Z. (2005) Bub1 multitasking in mitosis. Cell Cycle, 4 , 262-265. (PDF)
  8. Luo , X. and Yu, H. (2005) Purification and assay of Mad2: a two-state inhibitor of APC/C. Methods Enzymology , 398 , 246-255. (PDF)
  9. Qi , W. and Yu, H. (2005) The spindle checkpoint and chromosome stability. Genome Dynamics, 1 , 116-130.
  10. Yu, H. (2006) Structural activation of Mad2 in the mitotic spindle checkpoint: the “two-state Mad2” model versus the “Mad2 template” model. J. Cell Biol., 173 , 153-157. (PDF)
  11. Yu, H. (2007) Chk1: a double agent in cell cycle checkpoints. Dev. Cell, 12 , 167-168. (PDF)
  12. Yu, H. (2007) Cdc20: a WD40 activator for a cell cycle degradation machine. Mol. Cell, 27 , 3-16. (PDF)
  13. Kang, J. and Yu, H. in Checkpoint Responses in Cancer Therapy ; Dai, W.; Ed.; Cancer Drug Discovery and Development; Humana Press: Totowa, NJ, 2007, in press.
  14. Diaz-Martinez, L. and Yu, H. (2007) Running on a treadmill: dynamic inhibition of APC/C by the spindle checkpoint. Cell Division, 2 , 23.
  15. Yu, H. (2007) PP2A as a mercenary of warring kinases in the egg. Proc. Natl. Acad. Sci . U. S. A., 104 , 17245-17246. (PDF)
  16. Potts, P. R. and Yu, H. Chromosome formation in Wiley Encyclopedia of Chemical Biology ; Jon Wiley and Sons Inc.: Hoboken, NJ, 2008, doi : 10.1002/9780470048672.wecb650.
  17. Gocke , C. B. and Yu, H. (2008) Identification of SUMO targets through in vitro expression cloning. Methods Mol. Biol., in press.
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